5/6/2023 0 Comments Amadine thierry durandPatient and public involvement Patients and/or the public were not involved in the design, conduct, reporting or dissemination plans of this research. CM has been awarded the UEG Research Award 2020 for her stay at The University of Gothenburg and by the FARE Fellowship of the French Gastroenterology Society in 2015. GG, CP and SM received a scholarship from Ministère de l’Enseignement Supérieur, de la Recherche et de l’Innovation.Ĭompeting interests PP is a senior fellow of the Institut Universitaire de France. PP and GG received support from CNRS and University of Strasbourg. AS, PP and GG are supported by Centre National de la Recherche Scientifique. Funding from the Fédération pour la Recherche sur le Cerveau (FRC) FRC20200411001 for AS and NC. PLF and NC are responsible for the overall content as guarantor.įunding Funding from the Agence Nationale de la Recherche (ANR): ANR-18-CE14-0039 for N.C, ANR-20-CE14-0011 for NC & JBM, ANR-17-EURE-022 for EURIDOL Graduate School of Pain, ANR-11-INBS-0010 for Metabohub, ANR-11-EQPX-0003 for the platform Aninfimip. CM and GB provided patient samples and clinical scores and wrote the manuscript. HH and LD provided patient samples and clinical scores. GG, JB-M, AS, EO PB and GD designed the research studies and wrote the manuscript. JMG, AG and TD designed and synthesised the lipopeptides. GL, J-PM, PF, SL and AD acquired and analysed the data. AH, BA RM and FA conducted the experiments and acquired and analysed the data. SM, GP and MSdesigned the research studies, conducted the experiments, and acquired and analysed the data. 13 Department of Molecular and Clinical Medicine, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Swedenĭr Nicolas Cenac, INSERM, Toulouse CS 60039, Occitanie, France Ĭontributors CP, PLF and NC designed the research studies conducted the experiments and acquired, analysed the data and wrote the manuscript.12 Institute for Research and Innovation in Biomedicine, INSERM CIC-CRB 1404, INSERM UMR 1073, Normandy University, Rouen, France.11 Gastroenterology Department, Rouen University Hospital, Rouen, France.10 Laboratory of Intestinal Neuro-immune Interaction, Translational Research Center for Gastrointestinal Disorders, Department of Chronic Diseases, Metabolism and Ageing, KU Leuven, Leuven, Belgium.9 Metatoul-AXIOM Platform, MetaboHUB, Toxalim, INRAE, Toulouse, France.8 Toxalim (Research Center in Food Toxicology), Toulouse University, INRAE, ENVT, INP-Purpan, UPS, Toulouse, France.7 Service de bactériologie-hygiène, CHU Toulouse, Hôpital Purpan, Toulouse, France.6 Institut des Biomolécules Max Mousseron (IBMM), UMR 5247, CNRS, Université de Montpellier, ENSCM, Montpellier, France.5 INFINITY, Université de Toulouse-Paul Sabatier, INSERM, CNRS, UPS, Toulouse, France.4 Institut des Neurosciences Cellulaire et Integrative (INCI), Centre National de la Recherche Scientifique, Université de Strasbourg, Strasbourg, France.3 I2MC, Université de Toulouse, Inserm, Université Toulouse III – Paul Sabatier (UPS), Toulouse, France.2 Lipidomic, MetaboHUB-MetaToul, National Infrastructure of Metabolomics and Fluxomics, Toulouse, France.1 IRSD, Université de Toulouse-Paul Sabatier, INSERM, INRAe, ENVT, UPS, Toulouse, France.The synthesized metabolites were applied in targeted lipidomics to prove lipid peroxidation in edible oils of commercial nutraceuticals. The first one relied on a racemic approach to 18( RS)-18-F 3t-IsoP using an oxidative radical anion cyclization as a key step, whereas the second used an enzymatic deracemization of a bicyclooctene intermediate obtained from cyclooctadiene to pursue an asymmetric synthesis. Three new PUFA-metabolites, namely 18-F 3t-isoprostane (IsoP) from eicosapentaenoic acid (EPA), 20-F 4t-neuroprostane (NeuroP) from docosahexaenoic acid (DHA) and 20-F 3t-NeuroP from docosapentaenoic acid (DPA n-3) were synthesized by two complementary synthetic strategies. However, since a large number of isomeric metabolites is formed in parallel, their quantification remains difficult without primary standards. As potential biomarkers of OS, their in vivo quantification is of great interest. Oxidative stress (OS) is an in vivo process leading to free radical overproduction, which triggers polyunsaturated fatty acid (PUFA) peroxidation resulting in the formation of racemic non-enzymatic oxygenated metabolites.
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